Improved cytochemical method for cyclic 3',5'-nucleotide phosphodiesterase in rat rod outer segments.
نویسندگان
چکیده
منابع مشابه
Histochemical evidence of cyclic nucleotide phosphodiesterase in photoreceptor outer segments.
A histochemical demonstration of cyclic 3',5'-nucleotide phosphodiesterase in retinal photoreceptor outer segments is reported for the first time. The enzyme appears to be located on outer segment lamellae. It is not found in adjacent inner segment or nuclear regions. This histochemical localization is consistent with previously reported biochemical studies showing high phosphodiesterase activi...
متن کاملPurification and properties of the light-activated cyclic nucleotide phosphodiesterase of rod outer segments.
Frog (Rana catesbiana) rod outer segment disc membranes contain a cyclic nucleotide phosphodiesterase (EC 3.1.4.17) which is activated by light in the presence of ATP. This enzyme is firmly bound to the disc membrane, but can be eluted from the membrane with 10 mM Tris-HCl buffer, pH 7.4 and 2 mM EDTA. The eluted phosphodiesterase has reduced activity, but can be activated approximately 10-fold...
متن کاملCyclic GMP diffusion coefficient in rod photoreceptor outer segments.
Cyclic GMP (cGMP) is the intracellular messenger that mediates phototransduction in retinal rods. As photoisomerizations of rhodopsin molecules are local events, the longitudinal diffusion of cGMP in the rod outer segment should be a contributing factor to the response of the cell to light. We have employed the truncated rod outer segment preparation from bullfrog (Rana catesbeiana) and tiger s...
متن کاملPhagocytosis of outer segments by cultured rat pigment epithelium. Reduction by cyclic AMP and phosphodiesterase inhibitors.
Phagocytosis of isolated outer segments by cultured rat pigment epithelium was decreased by cyclic 3',5'adenosine monophosphate (cyclic AMP), dibutyryl cyclic AMP, and three phosphodiesterase inhibitors (isobutylmethylxanthine, papaverine, and SQ 65442). Cyclic GMP and its dibutyryl derivative had little effect on phagocytosis. Phagocytosis was moderately reduced by several other purine nucleot...
متن کاملCyclic-nucleotide Phosphodiesterase
Cyclic nucleotides have been implicated in the differentiation and function of the vertebrate retina. In the normal retina of DBA mice, the specific activity of cyclic-nucleotide phosphodiesterase (PDE), with cyclic-AMP as the substrate (cAMP-PDE), increases eightfold between the 6th and 20th postnatal day. Kinetic analysis of retinae from newborn mice reveals a PDE with a single Michaelis cons...
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ژورنال
عنوان ژورنال: ACTA HISTOCHEMICA ET CYTOCHEMICA
سال: 1992
ISSN: 0044-5991,1347-5800
DOI: 10.1267/ahc.25.697